We also study selected human proteins, especially WT1, AKIP1, DHX15, and NMDAR. For their production in bacterial, insect or tissue cells and subsequent isolation and purification, we engineer a broad spectrum of expression vectors. Using directed or alanine-scanning mutagenesis we identify the important amino acid residues or functional domains for their cellular functioning. The biochemical properties of purified proteins are tested using variety of biochemical or structural approaches.
Grant support: TAČR 2019-2020
Collaborations: Institute of Physiology CAS, Motol University Hospital