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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373
Czech Post certified digital mail code: sp4j9ch
Copyright: UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
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As the Department name indicates, the Department of Biotechnology is engaged in education, science, and consultancy activities in biotechnologies from the chemical, technical, and engineering points of view. The Department's core activities are in brewing, biotechnologies for environmental protection, alternative fuel production, issues of biofilms, and the production of biodetergents and enzymes.
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Interested students can participate in scientific and research work at the Department from the outset of their studies.
Given a wide range of contacts in Europe and worldwide, it is possible to apply for study or research fellowships.
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Research focuses on biotechnological processes in their entireties—including the behavior of biological agents; the study of unit operations which are involved in modern biotechnology, including downstream processing; aspects of bioengineering and the modeling and simulation of bioprocesses; the application of biotechnological processes in the food, pharmaceutical, biotechnology and chemical industries; and environmental protection.
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- Assoc. Prof. Martin Halecký, PhD (contact person)
- Prof. Tomáš Brányik, PhD
- Prof. Jan Páca, DSc
- Tomáš Potočár, MSc (doctoral student)
- Jiří Mach, MSc (doctoral student)
- Undergraduate students
In Laboratory of Bioengineering we deal with production and decontamination biotechnologies from the point of view of bioreactors and control of processes as well as from the point of view of (micro)biology, biochemistry and genetics. Using submerged and biofilm photobioreactors we study the application of unicellular marine and freshwater algae and photosynthesizing bacteria to produce biologically active substances. We use modern technologies of multispectral analysis and 3D scanning to study the potential of microorganisms and their products to produce biopreparations e.g. biostimulants and biopesticides for plant treatment in sustainable agriculture. We also focus on enzyme synthesis and recycling of sustainable polyurethane materials with maximum use of natural and renewable raw materials using modern concepts such as "Green chemistry", "Cradle-to-grave" and "Cradle-to-cradle". Using contact angle and zeta potential we study the surface properties of both biomass and packing materials, which we further use in the study of biofilm formation, flocculation, flotation and emulsification.
To fulfill our research, we use bioreactors and other equipment available at our department for cell cultivation, downstream processes, HPLC and GC analyses and special methods.
We cooperate with a number of Czech and foreign companies and universities, where our students can apply and mainly expand their knowledge and skills:
- The Leibniz Institute of Surface Engineering (IOM)
- University of Minho, Braga, Portugal
- KU Leuven, Belgium
- Escola de Engenharia de Lorena da Universidade de São Paulo, Brazil
- The Institute of Chemical Process Fundamentals of the ASCR, v. v. i., Czech Republic
- Institute of Microbiology of the ASCR, v. v. i., Czech Republic
- EcoFuel Laboratories, Czech Republic
- Research Institute of Brewing and Malting Inc., Czech Republic
- BOHEMIACHLAD Ltd., Czech republic
- Dekonta Inc., Czech Republic
- University of South Bohemia in České Budějovice, Czech Republic
- Faculty of Science, Charles University in Prague , Czech Republic
- Institute of Microbiology of the ASCR, v. v. i., Prague, Czech Republic
- Texas A&M University, Kingsville, TX, USA
- University of North Dakota, Grand Forks, ND, USA
- University of Louisiana at Lafayette, LA, USA
- Universidade da Coruña, La Coruña, Spain
- Instituto Superior Técnico, Lisboa, Portugal
Our research is supported not only by enterprises but also by research projects:
Biofiltration impregnated composite materials and substrates, FW01010370
Funding provider: Technology Agency of the Czech Republic (TACR) – programme TREND
Researcher in Charge: Ing. Luboš Zápotocký Ph.D. (Dekonta a.s.)
Co-researcher: Assoc. Prof. Martin Halecký PhD, Department of Biotechnology, UCT Prague
Period: 2020-2023
Use of wastes from dairies for production of new dairy products and dietary supplements with the addition of microalgae and their components, QK1910300
Funding provider: Ministry of Agriculture of the Czech Republic
Researcher in Charge: Prof. Tomáš Brányik PhD, Department of Biotechnology, UCT Prague
Participants: EcoFuel s.r.o., Ekomilk a.s., Ingredia s.r.o., VÚM s.r.o.
Period: 2019-2022
Microporous polyurethanes with adjustable rate of biodegradation, 19-08549S
Funding provider: Czech Science Foundation (GACR)
Researcher in Charge: Ing. Hynek Beneš PhD, DSc, Institute of Macromolecular Chemistry, Czech Academy of Sciences
Co-researcher: Assoc. Prof. Martin Halecký PhD, Department of Biotechnology, UCT Prague
Period: 2019-2021
Funding provider: Czech Science Foundation (GACR)
Researcher in charge: doc. RNDr. Martin Pivokonský Ph.D., Institute of Hydrodynamics of the Czech Academy of Sciences, Prague
Co-researcher: prof. Tomáš Brányik Ph.D., Department of Biotechnology, UCT Prague
Period: 2018-2020
Zuzka in Colombia at Universidad de San Buenaventura Cartagena (2020)
At the UCT's cottage in Krkonoše Mountains (2020) - On the Road
At the UCT's cottage in Krkonoše Mountains (2020) - Meal at the cottage - dron view
Festival of Science (2019)
Honza Caught Fish on Internship at Partner University of North Dakota, USA (2019)
“Lesamáj” in the Dejvice Campus (2018)
Krkonoše Mountains (2018)
Krkonoše Mountains - Conquest of Sněžka mountain (2018)
Honza and Maryna's Wedding - Group Photo (2018)
Honza and Maryna's Wedding - (Research) Groups Battle (2018)
Krkonoše Mountains (2017)
Honza Won on Dragon Boats (2013)
Functional materials for (bio)technologies
In collaboration with our research partners, we develop and test packing materials with various functional potentials combining specific surface properties e.g. specific surface area, hydrophobicity, pore size and distribution, sorption properties, mineral nutrient content, utilizable carbon and nitrogen content and inducers for microbial production of specific substances e.g. biopesticides. We test intentionally synthesized polyurethane foams with controlled biodegradability as packing materials for production and decontamination bioreactors (collaboration with Institute of Macromolecular Chemistry, Czech Academy of Sciences) and various types of carbonaceous materials e.g. biochars (collaboration with ENERGO Zlatá Olešnice and Institute of Energy of ICT Prague). We predict the suitability of materials as a biofilm carrier (X/DLVO theory and balance of interfacial free energy) and observe real surface colonization (microscopy and image analysis).
Bioactive compounds from marine microorganisms
Marine unicellular microorganisms are very rich in bioactive compounds and thus may be used in several biological applications. The most studied substances are polyunsaturated fatty acids (PUFAs), sterols, proteins and enzymes, and vitamins and pigments, which find applications in areas such as human and animal nutrition, therapeutics, and aquaculture. This project will focus mainly on marine microorganisms (e.g. algae, cyanobacteria, bacteria), their cultivation and culture condition-induced changes in the composition of biomass, and the identification of bioactive compounds with biotechnological potential.
Surface interactions of microalgae
This project will carry out experimental research of factors (nutritive, growth conditions) influencing adhesion of industrially promising microalgae (food, environmental processes) to solid surfaces. The project is based on comparison of experimental data on adhesion intensities of microalgae, grown under different conditions to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (DLVO theory, balance of interfacial free energies) using physicochemical properties of interacting surfaces. The results are applicable both in cases when suppression of algal adhesion is desired (inner wall of photobioreactors) and in processes (biodegradation, biotransformation) where algal biofilm formation (immobilization) is wanted.
Biopesticides
Biopesticides are a key response to increased environmental quality as well as food quality requirements. In cooperation with research partners we deal with chitinase-based biopesticides (colaboration with Dekonta a.s.) and Pythium oligandrum (colaboration with Biopreparáty, s.r.o.). Chitinase-based biopesticides can be used as insecticides and fungicides. We focus on optimizing production process using biofilm systems on specifically synthesized polyurethane foam carriers with controlled biodegradability. Pythium oligandrum can be used in medicine, as a biopesticide in agriculture, and as a prevention of mold development in the entire malt production chain in the food industry (prevention of mycotoxins contamination).
Technologies and (bio)reactors for wastewater and gas treatment
We are engaged in the use of packed bed and free-cell bioreactors for removal of volatile organic compounds (VOCs - petrol, diesel, mixtures of acetone and styrene and paint solvents and odorous substances) from waste gases and micropollutants (pharmaceuticals and pesticides) from contaminated water. In particular, we study the synergistic effects of the combination of physical-chemical and biological approaches such as multiphase bioreactors (absorption-biodegradation) and the combination of adsorption pre-columns with bioreactor or adsorption packing materials (adsorption - biodegradation).
Biosorption potential of phototrophic microorganisms
Microalgal cell surfaces display a variety of binding sites, which allow biosorption of biologically active compounds. However, little is known about biosorption potential of phototrophic microorganisms towards organic molecules. The project aims to clarify fundamental relationships between adsorbent composition (microalgae and its fractions) and chemical structure of adsorbate. The scope of experimental work is defined by phototrophic taxons (Chlorella, Porphyridium, Arthrospira) and series of target compounds (polyphenols) with varying structures. Data on biosorption of individual polyphenols and their mixtures will be supported by physicochemical and chemical characterization of interacting entities, rheological data on dispersed systems and mathematical description of reaction equilibrium and kinetics. Based on this information, experiments will be performed to test 1) biosorption of ecologically, nutritionally, therapeutically relevant compounds of microalgal biomass or its fractions, 2) effective separation methods of dispersed sorbents from solutions, and 3) scale-up approaches.
Potočár T., Pereira J.A.V., Brányiková I., Barešová M., Pivokonský M., Brányik T.: Alkaline flocculation of Microcystis aeruginosa induced by calcium and magnesium precipitates. Journal of Applied Phycology, 32, 329–337(2020) IF 2,635 (on-line)
Halecký M., Kozliak E.: Modern Bioremediation Approaches: Use of Biosurfactants, Emulsifiers, Enzymes, Biopesticides, GMOs, in Advanced Nano-Bio Technologies for Water and Soil Treatment, Chapter: Biotechnologies for Soil Treatment, 2020, Springer International Publishing, Basel, Switzerland ISBN 978-3-030-29839-5, DOI: 10.1007/978-3-030-29840-1 (on-line)
Chalupa J., Pocik O., Halecky M., Kozliak E.: Thermophilic waste air treatment of an airborne ethyl acetate/toluene mixture in a bubble column reactor: Stability towards temperature changes, Journal of Hazardous Materials, Journal of Hazardous Materials 384, (2020) IF 7,65; Q1 (on-line)
Branyikova I., Filipenska M., Urbanova K., Ruzicka M. C., Pivokonsky M., Branyik T.: Physicochemical approach to alkaline flocculation of Chlorella vulgaris induced by calcium phosphate precipitates. Colloids and Surfaces B: Biointerfaces 166, 54-60 (2018), IF 3,997 (on-line)
Jan Chalupa - Best Student Poster (2017)
Patent
Branyik T., Humhal T., Kastanek P. (2017) Cultivating sea protist biomass, preferably microorganisms of Thraustochytriales, by performing cultivation in cultivation medium comprising waste brine solution from process of demineralization of sweet whey. European Patent Number: EP2990474-A1 (on-line)
Baresova M., Pivokonsky M., Novotna K., Naceradska J., Branyik T.: An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Research 122, 70-77 (2017). ISSN: 0043-1354, IF 6,942. (on-line)
Vanek T., Silva A., Halecky M., Paca J., Ruzickova I., Kozliak E., Jones K.: Biodegradation of airborne acetone/styrene mixtures in a bubble column reactor. J. Environ. Sci. Health Part A-Toxic/Hazard. Subst. Environ. Eng. 52(9):905-915 (2017) (on-line)
Award Visionaries 2016 - Tomáš Brányik a Tomáš Humhal (2016)
Šafařík I., Procházková G., Pospíšková K., Brányik T.: Magnetically modified microalgae and their applications. Critical Reviews in Biotechnology 36(5), IF = 7,178. (2016) (on-line)
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- practical experimental work with different kinds of (bio)reactors
- measurement and control of (bio)processes
- cultivation techniques of mesophilic and thermophilic bacteria as well as yeasts, molds and freshwater and marine unicellular algae and photosynthetic bacteria
- practical experimental work with HPLC and GC systems
- determination of enzyme activity, advanced cell staining, biofilm formation kinetics (image analysis) and other practical microbiological and biochemical methods
- surface characterization techniques including processing and interpretation of results using (X)DVLO theory
- design and analysis of experiments and balancing and mathematical description of (bio)processes
Youngest chemists
3D chromatogram of the determination of micropolutants (pesticides) in water
Trickling biofilter for waste air treatment
Chlorella algae cultivation in photobioreactors
Fusarium solani on Rose Bengal agar
We are Involved in Teaching of Bioengineering and Biotechnology subjects:
- Bioengineering I (in Czech)
- Bioengineering II (in Czech)
- Bioengineering (in English)
- Basics of Bioengineering (in Czech)
- Processes and Operations in Brewing Production (in Czech)
- Trends in Biotechnologies (in English)
- Cultivation Techniques and Bioprocess Modeling (in Czech)
- Simultaneous degradation of acetone and styrene in a continuously operating submerged reactor (Laboratory task)
- Heterotrophic Algae Cultivation (Laboratory Task)
- Bioprocess Modeling (lecture)