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Microbiological Methods

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Microscopy

  • Fluorescence microscope Nikon Eclipse E 400
  • Fluorescence microscope Olympus BX40
  • Stereomicroscope Nikon SMZ 800
  • Image analysis - software NIS-Elements AR 3.0 (Laboratory Imaging)

 

Flow Cytometry

Cytometr Partec PAS III (Partec, GmbH SRN) – light source – 488 nm argon-ion laser and mercury lamp HBO

  • Cell sorter
  • Measurement of FSC, SSC, fluorescence FL1, FL2, FL3, FL6
  • Analysis of physiological state of bacteria and yeasts
  • Cell cycle
  • Intracellular cell components – glycogen, DNA, proteins, trehalose

Bioscreen C

  • Long-term cultivation in the micro-scale
  • Quantitative spectrophotometric analysis

 

Supporting Equipment

  • Laminar boxes
  • Anaerobic box
  • Freezers (-70 °C)
  • Steam autoclaves (2 x AUT 26 Chirana, Tuttnauer ELV 3170 a ELV 3870)
  • Shaking machines (aerobic/anaerobic, -15-80°C)
Updated: 21.5.2015 15:15, Author: Martin Halecký

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