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UCT PragueFFBTDepartment of Biotechnology About department Equipment
 
Cultivation Equipment

Cultivation Equipment

For the cultivation of microorganisms, we have a wide range of bioreactors for both aerobic and anaerobic cultivation, equipped with on-line measurement and control of pH, O2 in gases, dissolved O2, redox potential, CO2, temperature, etc.

Submerged Reactors

  • bubble columns for waste air/water treatment
  • B-Braun stirred reactors, 0.5–6 L, including control units (2 units)
  • Labfors stirred reactors, 3.5 L (2 units) and 7.5 L (1 unit), including control units
  • Applikon MiniBio 250 mL V2 stirred reactors (4 units), including control units
  • photobioreactors – a cultivation set of illuminated bubbled cylindrical reactors; Minitron illuminated shaker (cultivation in flasks or on Petri dishes)
  • OxiTop C with OxiTop Control OC 100

Packed-Bed Reactors

  • various types of biofilters for waste air treatment
  • various types of biofilters for wastewater treatment
  • biotrickling filters for waste air treatment
Microbial and Tissue Cultures

Microbial and Tissue Cultures

Microscopy

  • Nikon Eclipse E 400 fluorescence microscope
  • Olympus IX fluorescence microscope
  • Olympus BX40 fluorescence microscope
  • Olympus CKX31 inverted light microscope
  • Nikon SMZ 800 stereomicroscope
  • image analysis – NIS-Elements AR 3.0 software (Laboratory Imaging)

Flow Cytometry

  • BD Aria III flow cytometer with cell sorter

Spectrophotometers and Microplate Readers

  • BIOSCREEN C
  • Infinite M200PRO
  • Hach Lange DR 5000
  • Biochrom Libra S22

Other Equipment

  • laminar flow cabinets
  • anaerobic chamber
  • CO2 incubators
  • Cell Counter II FL
  • Thermo Heto PowerDry LL3000 freeze dryer
  • ultra-low temperature freezers (−70 °C)
  • steam autoclaves (2× AUT 26 Chirana, Tuttnauer ELV 3170 and ELV 3870)
  • shakers (aerobic/anaerobic, −15 to 80 °C)
Downstream Processing

Downstream Processing

Distillation and Rectification

  • Hempel packed distillation column with a distillation head for reflux take-off and a 4 L heating mantle with a 4 L distillation flask
  • Marcusson distillation apparatus

Centrifugation

  • Sigma 1K15 laboratory centrifuge
  • Hettich Mikro 220R laboratory centrifuge
  • Selecta Medifriger-BL laboratory centrifuge
  • Janetzki K26 laboratory centrifuge
  • Heraeus Megafuge 16R laboratory centrifuge
  • Jouan CR3 laboratory centrifuge
  • Mechanika Precyzyjna MPW-330 laboratory centrifuge

Gas Stripping

  • Stripping apparatus with stripping-gas recycle driven by a Secoh MK10 diaphragm pump, with the option of cold trapping or adsorption on activated carbon. Various stripping columns, or a laboratory fermenter directly, can be connected to the apparatus. Temperature is controlled by a Julabo ED 5 thermostat and a Julabo MC F25 cryostat.
Chromatographic Analysis

Chromatographic Analysis

Gas Chromatography

GC-MS: Agilent 8890 GC System (MSD 5977B + PAL3 RSI autosampler), Agilent 8890 GC System (MSD 5977C + PAL3 RSI Series II autosampler)

Both systems use PAL3 RSI for automated liquid injection, static and dynamic headspace, and SPME (solid-phase microextraction).

GC-FID, GC-FPD and GC-TCD (Agilent 5890, 6890N)

  • Qualitative and quantitative analysis of volatile pollutants in the gas phase (volatile organic compounds – VOCs)
  • Qualitative and quantitative analysis of volatile organic compounds in alcoholic and non-alcoholic beverages (beer, wine, spirits)
  • Qualitative and quantitative analysis of volatile organic compounds in food raw materials (hop essential oils, tropical fruit, citrus peel)

High-Performance Liquid Chromatography

Shimadzu LCMS-8050 liquid chromatograph mass spectrometer; Agilent HPLC series 1100, 1200 and 1260 Infinity II (photodiode array detector – PDA, refractive index detector – RI, fraction collector, autosamplers); Watrex HPLC + DAD (Thermo UV6000), RI (KNAUER K2300)

  • Qualitative and quantitative analysis of organic pollutants in water, soil and sediments (nitroaromatics, nitroesters, phenol and its derivatives)
  • Qualitative and quantitative analysis of organic compounds in alcoholic and non-alcoholic beverages (saccharides, amino acids, iso-α-acids, polyphenols)
  • Qualitative and quantitative analysis of organic compounds in food raw materials (hops – hop resins; barley and malt – polyphenols)
  • Qualitative and quantitative analysis of organic compounds in studies of cells and biofilm formation (fatty acids)
  • Determination of saccharides, alcohols and organic acids in liquid samples

Low-Pressure Liquid Chromatography

Bio-Rad BioLogic LP with BioFrac Fraction Collector

  • purification of proteins, peptides and other biomolecules
  • fraction collection

Ion Chromatography

HPLC-IC + Shodex CD5 conductivity detector

  • Qualitative and quantitative analysis of cations (Li+, Na+, NH4+, K+, Ca2+, Mg2+)
  • Qualitative and quantitative analysis of anions (F−, Cl−, NO2−, Br−, NO3−, PO43−, SO42−)
Biopolymer Analysis

Biopolymer Analysis

Protein Analysis

  • vertical electrophoresis (Bio-Rad)
  • western blot (Bio-Rad)
  • cooled CCD camera (Fusion Fx, Vilber Lourmat)
  • microplate washer (HydroFlex, Tecan Life Sciences)
  • multimode microplate reader (Infinite M200PRO, Tecan Life Sciences)
  • medium-pressure liquid chromatograph (ÄKTA pure 25, GE Healthcare Life Sciences)
  • ultracentrifuge (Optima XPN-90, Beckman Coulter)
  • Differential scanning fluorimetry nanoDSF (Prometheus™ Panta) – an analytical system combining nanoDSF with dynamic and static light scattering (DLS/SLS) for comprehensive characterisation of protein stability
  • Hybrid microplate reader (SpectraMax iD5) – a multimode reader combining five modes: absorbance, fluorescence (top and bottom), luminescence, time-resolved fluorescence (TRF) and fluorescence polarisation

PCR Cyclers – DNA Synthesis and Amplification

  • Bio-Rad T100™ PCR cycler
  • Life Eco PCR cycler
  • Applied Biosystems™ QuantStudio™ 5 real-time PCR cycler, 96-well format

Qualitative and Quantitative RNA/DNA Analysis

  • Horizontal electrophoresis: Owl™ EasyCast™ B1A Gel Electrophoresis Systems
  • Quantum ST5 UV/VIS gel documentation system (Vilber Lourmat)
  • DeNovix DS-11 FX spectrophotometer and fluorometer
  • Agilent 2100 Bioanalyzer
Special Methods

Special Methods

Nibem-T (Haffmans)

Measurement of beer foam stability.

Krüss DSA30 Goniometer

Automatic determination of contact angles and, based on the Young–Laplace equation, of the surface and interfacial tension of solid surfaces or of cells deposited as a thin layer. These data can be used, for example, to calculate the free interaction energy of cell adhesion.

Multifunctional Scanning System for Multispectral Analysis and 3D Scanning of Plants

A unique scanning system (AR4 robotic arm equipped with a JAI FS-3200D-10GE multispectral camera with a JVS-C118-0824-C3 lens, a Revopoint POP 3 3D scanner and a DAHENG IMAGING MER2-1220-32U3C camera with an LCM-12MP-06MM-F2.4-1.7-ND1 lens) for non-destructive plant analysis in research on biostimulants and biopesticides.

MicroCal PEAQ-ITC

The MicroCal PEAQ-ITC is a highly sensitive isothermal titration calorimeter for the direct determination of the thermodynamic parameters of biomolecular interactions in their native state. It measures binding affinity (KD), stoichiometry (n) and enthalpy (ΔH) simultaneously in a single experiment, providing a complete thermodynamic profile of the interaction (including the calculation of entropy ΔS and Gibbs free energy ΔG) without the need for fluorescent labelling or sample immobilisation.