stdClass Object
(
[nazev] => Department of Biotechnology
[adresa_url] =>
[api_hash] =>
[seo_desc] =>
[jazyk] =>
[jednojazycny] =>
[barva] =>
[indexace] => 1
[obrazek] =>
[ga_force] =>
[cookie_force] =>
[secureredirect] =>
[google_verification] =>
[ga_account] =>
[ga_domain] =>
[ga4_account] => G-VKDBFLKL51
[gtm_id] =>
[gt_code] =>
[kontrola_pred] =>
[omezeni] => 0
[pozadi1] =>
[pozadi2] =>
[pozadi3] =>
[pozadi4] =>
[pozadi5] =>
[robots] =>
[htmlheaders] =>
[newurl_domain] => 'ub.vscht.cz'
[newurl_jazyk] => 'en'
[newurl_akce] => '[en]'
[newurl_iduzel] =>
[newurl_path] => 8548/5338/5340
[newurl_path_link] => Odkaz na newurlCMS
[iduzel] => 5340
[platne_od] => 31.10.2023 17:10:00
[zmeneno_cas] => 31.10.2023 17:10:18.371543
[zmeneno_uzivatel_jmeno] => Jan Kříž
[canonical_url] =>
[idvazba] => 6020
[cms_time] => 1713246626
[skupina_www] => Array
(
)
[slovnik] => stdClass Object
(
[logo_href] => /
[logo] => 
[social_fb_odkaz] =>
[social_tw_odkaz] =>
[social_yt_odkaz] =>
[paticka_budova_a_nadpis] => BUILDING A
[paticka_budova_a_popis] => Rector,
Department of Communications,
Department of Education,
FCT Dean’s Office,
Centre for Information Services
[paticka_budova_b_nadpis] => BUILDING B
[paticka_budova_b_popis] => Department of R&D, Dean’s Offices:
FET,
FFBT,
FCE,
Computer Centre,
Department of International Relations,
Bursar
[paticka_budova_c_nadpis] => BUILDING C
[paticka_budova_c_popis] => Crèche Zkumavka,
General Practitioner,
Department of Economics and Management,
Department of Mathematics
[paticka_budova_1_nadpis] => NATIONAL LIBRARY OF TECHNOLOGY
[paticka_budova_1_popis] =>
[paticka_budova_2_nadpis] => CAFÉ CARBON
[paticka_budova_2_popis] =>
[paticka_adresa] => UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373
Czech Post certified digital mail code: sp4j9ch
Copyright: UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
[paticka_odkaz_mail] => mailto:Martin.Halecky@vscht.cz
[social_fb_title] =>
[social_tw_title] =>
[social_yt_title] =>
[google_search] => 001523547858480163194:u-cbn29rzve
[drobecky] => You are here: UCT Prague – FFBT – UB
[autor] => Author
[aktualizovano] => Updated
[paticka_mapa_alt] =>
[intranet_odkaz] => http://intranet.vscht.cz/
[intranet_text] => Intranet
[den_kratky_4] => Thurs
[novinky_kategorie_1] => UCT Events
[novinky_kategorie_2] => Important Dates
[novinky_kategorie_3] => Student Events
[novinky_kategorie_4] => Fun
[novinky_kategorie_5] => Science
[novinky_archiv_url] => /news
[novinky_servis_archiv_rok] => Annual Archive
[novinky_servis_nadpis] => News Settings
[novinky_dalsi] => More News
[den_kratky_5] => Fri
[archiv_novinek] => Annual Archive
[logo_mobile_href] => /
[logo_mobile] =>
[mobile_over_nadpis_menu] => Menu
[mobile_over_nadpis_search] => Search
[mobile_over_nadpis_jazyky] => Languages
[mobile_over_nadpis_login] => Login
[menu_home] => Homepage
[zobraz_desktop_verzi] => switch to desktop version
[novinky_archiv] => News Archive
[zobraz_mobilni_verzi] => switch to mobile version
[zobrazit_kalendar] => Read Calendar
[paticka_mapa_odkaz] =>
[nepodporovany_prohlizec] => For full access, please use different browser.
[den_kratky_2] => Tue
[preloader] => Wait a second...
[den_kratky_6] => Sat
[den_kratky_1] =>
[den_kratky_0] =>
[den_kratky_3] =>
[social_in_odkaz] =>
[hledani_nadpis] => hledání
[hledani_nenalezeno] => Nenalezeno...
[hledani_vyhledat_google] => vyhledat pomocí Google
[social_li_odkaz] =>
)
[poduzel] => stdClass Object
(
[5343] => stdClass Object
(
[obsah] =>
[poduzel] => stdClass Object
(
[7900] => stdClass Object
(
[obsah] =>
[iduzel] => 7900
[canonical_url] => //ub.vscht.cz
[skupina_www] => Array
(
)
[url] => /[menu-top]
[sablona] => stdClass Object
(
[class] => api_kalendar
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[7902] => stdClass Object
(
[obsah] =>
[iduzel] => 7902
[canonical_url] => //ub.vscht.cz
[skupina_www] => Array
(
)
[url] => /[menu-jazyky]
[sablona] => stdClass Object
(
[class] => api_kalendar
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[7901] => stdClass Object
(
[obsah] =>
[iduzel] => 7901
[canonical_url] => //ub.vscht.cz
[skupina_www] => Array
(
)
[url] => /[menu-main]
[sablona] => stdClass Object
(
[class] => api_kalendar
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
)
[iduzel] => 5343
[canonical_url] => //ub.vscht.cz/5343
[skupina_www] => Array
(
)
[url] => /[en]/5343
[sablona] => stdClass Object
(
[class] => api_kalendar
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[5344] => stdClass Object
(
[obsah] =>
[poduzel] => stdClass Object
(
[7908] => stdClass Object
(
[nazev] =>
[seo_title] =>
[seo_desc] =>
[autor] =>
[autor_email] =>
[obsah] =>
[iduzel] => 7908
[canonical_url] => //ub.vscht.cz/home
[skupina_www] => Array
(
)
[url] => /home
[sablona] => stdClass Object
(
[class] => stranka_novinky
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[7909] => stdClass Object
(
[nazev] => Department of Biotechnology
[seo_title] => Department of Biotechnology
[seo_desc] =>
[autor] =>
[autor_email] =>
[obsah] => 
As the Department name indicates, the Department of Biotechnology is engaged in education, science, and consultancy activities in biotechnologies from the chemical, technical, and engineering points of view. The Department's core activities are in brewing, biotechnologies for environmental protection, alternative fuel production, issues of biofilms, and the production of biodetergents and enzymes.
[iduzel] => 7909
[canonical_url] => //ub.vscht.cz/department
[skupina_www] => Array
(
)
[url] => /department
[sablona] => stdClass Object
(
[class] => stranka_submenu
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[17822] => stdClass Object
(
[nazev] => Study
[seo_title] => Study
[seo_desc] =>
[autor] =>
[autor_email] =>
[perex] =>
[ikona] =>
[obrazek] =>
[obsah] => Our Department offers higher education in fields of traditional as well as modern biotechnologies, e.g. chemical and pharmaceutical industries and environmental protection, and bioengineering. The Department is accredited to provide three-year Bachelor programmes, two-year Master programmes, and Doctoral programmes.
Interested students can participate in scientific and research work at the Department from the outset of their studies.
Given a wide range of contacts in Europe and worldwide, it is possible to apply for study or research fellowships.
[iduzel] => 17822
[canonical_url] => //ub.vscht.cz/study
[skupina_www] => Array
(
)
[url] => /study
[sablona] => stdClass Object
(
[class] => stranka_submenu
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[17821] => stdClass Object
(
[nazev] => Research
[seo_title] => Research
[seo_desc] =>
[autor] =>
[autor_email] =>
[perex] =>
[ikona] =>
[obrazek] =>
[obsah] => 
Research focuses on biotechnological processes in their entireties—including the behavior of biological agents; the study of unit operations which are involved in modern biotechnology, including downstream processing; aspects of bioengineering and the modeling and simulation of bioprocesses; the application of biotechnological processes in the food, pharmaceutical, biotechnology and chemical industries; and environmental protection.
[iduzel] => 17821
[canonical_url] => //ub.vscht.cz/research
[skupina_www] => Array
(
)
[url] => /research
[sablona] => stdClass Object
(
[class] => stranka_submenu
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[17820] => stdClass Object
(
[nazev] => Cooperation and Partners
[seo_title] => Cooperation and Partners
[seo_desc] =>
[autor] =>
[autor_email] =>
[perex] =>
[ikona] =>
[obrazek] =>
[obsah] => 
Collaboration with domestic and international industrial and research partners on a wide range of joint projects involving basic and applied research, scaling of technologies and reactors, solution of technological problems by form of grants or cooperation contracts is an essential part of our scientific and research work. An integral part of this cooperation is the involvement of students of all learning levels in such projects to improve their knowledge and skills.
[iduzel] => 17820
[canonical_url] => //ub.vscht.cz/cooperation-partners
[skupina_www] => Array
(
)
[url] => /cooperation-partners
[sablona] => stdClass Object
(
[class] => stranka_submenu
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
[24134] => stdClass Object
(
[obsah] =>
[iduzel] => 24134
[canonical_url] =>
[skupina_www] => Array
(
)
[url] =>
[sablona] => stdClass Object
(
[class] =>
[html] =>
[css] =>
[js] =>
[autonomni] =>
)
)
)
[iduzel] => 5344
[canonical_url] => //ub.vscht.cz/5344
[skupina_www] => Array
(
)
[url] => /[en]/5344
[sablona] => stdClass Object
(
[class] => api_kalendar
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
)
)
[sablona] => stdClass Object
(
[class] => web
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
[api_suffix] =>
)
DATA
stdClass Object
(
[nazev] => Laboratory of Microbial Processes
[seo_title] => Laboratory of Microbial Processes
[seo_desc] =>
[autor] =>
[autor_email] =>
[perex] =>
[ikona] =>
[obrazek] =>
[ogobrazek] =>
[pozadi] =>
[obsah] => 
The main topics:
- Aerobic and anaerobic cultivation of bacteria, yeasts, and filamentous fungi
- Production of primary and secondary metabolites
- Characterization of microbial populations by use of fluorescent techniques
- Molecular biology of solventogenic clostridia
- Production of secondary metabolites by the fungus Monascus
Staff
Main research topics
Production of biofuels and bioplastics
Biotechnological production of biofuels (ethanol and butanol) and bioplastics precursors (succinic acid, lactic acid) by using first and second generation feedstocks. Design of fermentation process, selection of suitable feedstock, cultivation conditions and process arrangements (batch, fed-batch, continuous) for maximum yield and process productivity.
Molecular biology of solventogenic clostridia
Analysis of gene function and comparative genome studies focused on the basic research of the organic solvents biosynthesis during acetone–butanol–ethanol (ABE) fermentation. Methods for genetic manipulation of solventogenic clostridia development, qPCR based gene expression monitoring and description of the biobutanol biosynthesis on the molecular level. Endospore formation research of Clostridium bacteria.
Analysis of microbial populations on the single cell level
Detailed analysis of microbial populations using fluorescent microscopy, flow cytometry, and fluorimetry. Analysis of cell viability, growth rate, enzyme activities, concentration of intracellular compounds (DNA, NA, proteins, PHA, GFP, …) and other cell functions using selective staining with fluorescent probes. Measurement of these parameters for bacterial and yeast populations within the cultivation process under the influence of stress factors and antimicrobial agents.
Production of recombinant proteins by microbial host strains
Use of continuous processed data for the design of fed-batch processes for high cell density cultivation for production of recombinant proteins. Analysis of online and offline variables, design of suitable process arrangement.
Processing of waste materials from agriculture and industry into value-added products
Physico-chemical and/or enzymatic processing of lignocellulosic materials (wheat, wood, etc.) and keratin rich material (e.g. chicken feathers) and use of substrates based on nutrients derived from these feedstocks for biotechnological production of value-added products, e.g. biofuels (bioethanol, biobutanol), precursors of bioplastics (lactic and succinic acids), and others.
Production of secondary metabolites
Production of oligoketide pigments by Monascus purpureus by solid state or submersed cultivation. Optimization of cultivation conditions for preferential biosynthesis of yellow pigments (monascin and ankaflavin). Isolation and determination of pigments, citrinin, and monacolin K. Testing the effects of yellow pigments on the rat metabolic syndrome (in cooperation with ICEM).
Cooperation
- Zurich University of Applied Science, Swiss
- University of Insubria, Varese, Italy
- Institute of Nuclear and New Energy Technology, China
- Huazhong Agricultural University, Wuhan, China
- Auburn University, USA
- Ústav biomedicínského inženýrství, VUT Brno, Czech Republic
- Institute of Microbiology of the ASCR, v. v. i., Praha, Czech Republic
- Lonza Biotech, Kouřim, Czech Republic
- The Crop Research Institute, Praha, Czech Republic
[submenuno] =>
[urlnadstranka] =>
[newurl_domain] => 'ub.vscht.cz'
[newurl_jazyk] => 'en'
[newurl_akce] => '/research/groups/microbial'
[newurl_iduzel] => 17848
[newurl_path] => 8548/5338/5340/5344/17821/17845/17848
[newurl_path_link] =>
Odkaz na newurlCMS
[iduzel] => 17848
[platne_od] => 31.05.2023 12:35:00
[zmeneno_cas] => 31.05.2023 12:35:01.13112
[zmeneno_uzivatel_jmeno] => Martin Halecký
[canonical_url] =>
[idvazba] => 22067
[cms_time] => 1713246626
[skupina_www] => Array
(
)
[slovnik] => Array
(
)
[poduzel] => stdClass Object
(
[18068] => stdClass Object
(
[nazev] => Selected Results
[barva_pozadi] => cervena
[uslideru] => false
[text] =>
Patáková P. et al., Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level, Scientific Reports 9, 1371, 2019 (on-line)
Sedlar K. et al., Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq. BMC Genomics 19 (1), 415, 2018 (online)
Branska B. et al., Flow cytometry analysis of Clostridium beijerinckii NRRL B-598 populations exhibiting different phenotypes induced by changes in cultivation conditions. Biotechnology for Biofuels 11:99, 2018 (online)
Patakova P., Kolek J., Sedlář K., Koscova P., Branska B., Kupkova K., Paulova P., Provazník I.: Comparative analysis of high butanol tolerance and production in clostridia, Biotechnology Advances 36(3),721-738, 2018 (online)
Sedlar K., Kolek J., Provaznik I., Patakova P.: Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598. Journal of Biotechnology 244(20), 1-3 (2017) (online)
Kolek J., Sedlar K., Provaznik I., Patakova P. Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation and sonoporation. Biotechnology for Biofuels (2016) 9:14.DOI 10.1186/s13068-016-0436-y, (open access)
Paulová L., Patáková P., Branská B., Rychtera M., Melzoch K.: Lignocellulosic ethanol: Technology design and its impact on process efficiency. Biotechnology Advances. doi:10.1016/j.biotechadv.2014.12.002 (online)
Kolek J., Patakova P., Melzoch K., Sigler K., Řezanka T. Changes in membrane plasmalogens of Clostridium pasteurianum during butanol fermentation as determined by lipidomic analysis. PLOS ONE 10.1371/journal.pone.0122058 (2015)
Patáková P., Linhová M., Vykydalová P., Branská B., Rychtera M., Melzoch K.: Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia. Anaerobe 29: 113-117 (2014), ISSN 1075-9964, IF 2,022 (online)
Paulova L., Hyka P., Branska B., Melzoch K., Kovar K.: Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut(+). Journal of Biotechnology 157 (1): 180-188 (2012). (online)
Patáková P.: Monascus secondary metabolites: production and biological activity. Journal of Industrial Microbiology and Biotechnology 40(2): 169-181. (2013) (online)
Paulová L., Patáková P., Rychtera M., Melzoch K.: High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw. Fuel 122: 294-300. (2014) IF 3,406 (online)
[poduzel] => Array
(
)
[iduzel] => 18068
[canonical_url] =>
[skupina_www] => Array
(
)
[url] =>
[sablona] => stdClass Object
(
[class] => infobox
[html] =>
[css] =>
[js] =>
[autonomni] => 0
)
)
)
[sablona] => stdClass Object
(
[class] => stranka_obrazek_vertical
[html] =>
[css] =>
[js] =>
[autonomni] => 1
)
[api_suffix] =>
)
Selected Results
Patáková P. et al., Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level, Scientific Reports 9, 1371, 2019 (on-line)
Sedlar K. et al., Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq. BMC Genomics 19 (1), 415, 2018 (online)
Branska B. et al., Flow cytometry analysis of Clostridium beijerinckii NRRL B-598 populations exhibiting different phenotypes induced by changes in cultivation conditions. Biotechnology for Biofuels 11:99, 2018 (online)
Patakova P., Kolek J., Sedlář K., Koscova P., Branska B., Kupkova K., Paulova P., Provazník I.: Comparative analysis of high butanol tolerance and production in clostridia, Biotechnology Advances 36(3),721-738, 2018 (online)
Sedlar K., Kolek J., Provaznik I., Patakova P.: Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598. Journal of Biotechnology 244(20), 1-3 (2017) (online)
Kolek J., Sedlar K., Provaznik I., Patakova P. Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation and sonoporation. Biotechnology for Biofuels (2016) 9:14.DOI 10.1186/s13068-016-0436-y, (open access)
Paulová L., Patáková P., Branská B., Rychtera M., Melzoch K.: Lignocellulosic ethanol: Technology design and its impact on process efficiency. Biotechnology Advances. doi:10.1016/j.biotechadv.2014.12.002 (online)
Kolek J., Patakova P., Melzoch K., Sigler K., Řezanka T. Changes in membrane plasmalogens of Clostridium pasteurianum during butanol fermentation as determined by lipidomic analysis. PLOS ONE 10.1371/journal.pone.0122058 (2015)
Patáková P., Linhová M., Vykydalová P., Branská B., Rychtera M., Melzoch K.: Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia. Anaerobe 29: 113-117 (2014), ISSN 1075-9964, IF 2,022 (online)
Paulova L., Hyka P., Branska B., Melzoch K., Kovar K.: Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut(+). Journal of Biotechnology 157 (1): 180-188 (2012). (online)
Patáková P.: Monascus secondary metabolites: production and biological activity. Journal of Industrial Microbiology and Biotechnology 40(2): 169-181. (2013) (online)
Paulová L., Patáková P., Rychtera M., Melzoch K.: High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw. Fuel 122: 294-300. (2014) IF 3,406 (online)
