Wait a second...
Nepřihlášený uživatel
You are here: UCT PragueFFBTUB → Research → Research Groups → Laboratory of Bioprocess Optimization and Modeling
iduzel: 20044
idvazba: 24731
šablona: stranka_obrazek_vertical
čas: 28.3.2024 21:19:55
verze: 5378
uzivatel:
remoteAPIs:
branch: trunk
Server: 147.33.89.150
Obnovit | RAW
iduzel: 20044
idvazba: 24731
---Nová url--- (newurl_...)
domena: 'ub.vscht.cz'
jazyk: 'en'
url: '/research/groups/bioprocess'
iduzel: 20044
path: 8548/5338/5340/5344/17821/17845/20044
CMS: Odkaz na newurlCMS
branch: trunk
Obnovit | RAW

Laboratory of Bioprocess Optimization and Modeling

lom_vertical

Our laboratory engages with engineering and biological aspects of biotechnologies, especially in the area of processes optimization (e.g. media composition, culture conditions) and mathematical modeling of bioprocesses. The main research topics include:

  • Harnessing the biosorption potential of phototrophic microorganisms (study of mechanism and quantification of sorption of organic molecules)
  • Biotechnology of marine organisms (cultivation processes, utilization of waste water)
  • Study of the mechanism of adhesion of microbial contaminants of food to solid substrates
  • Biotechnology of microalgae (culture techniques, utilization of waste water, new methods of harvesting algae, adhesion of algae to surfaces)

Research group members

Main research topics

Biosorption potential of phototrophic microorganisms

 Microalgal cell surfaces display a variety of binding sites, which allow biosorption of biologically active compounds. However, little is known about biosorption potential of phototrophic microorganisms towards organic molecules. The project aims to clarify fundamental relationships between adsorbent composition (microalgae and its fractions) and chemical structure of adsorbate. The scope of experimental work is defined by phototrophic taxons (Chlorella, Porphyridium, Arthrospira) and series of target compounds (polyphenols) with varying structures. Data on biosorption of individual polyphenols and their mixtures will be supported by physicochemical and chemical characterization of interacting entities, rheological data on dispersed systems and mathematical description of reaction equilibrium and kinetics. Based on this information, experiments will be performed to test 1) biosorption of ecologically, nutritionally, therapeutically relevant compounds of microalgal biomass or its fractions, 2) effective separation methods of dispersed sorbents from solutions, and 3) scale-up approaches.

Bioactive compounds from marine microorganisms

Marine unicellular microorganisms are very rich in bioactive compounds and thus may be used in several biological applications. The most studied substances are polyunsaturated fatty acids (PUFAs), sterols, proteins and enzymes, and vitamins and pigments, which find applications in areas such as human and animal nutrition, therapeutics, and aquaculture. This project will focus mainly on marine microorganisms (e.g. algae, cyanobacteria, bacteria), their cultivation and culture condition-induced changes in the composition of biomass, and the identification of bioactive compounds with biotechnological potential.

Anaerobic food spoiling bacteria and their biofilm forming potential

The project is aimed at isolation and identification of anaerobic bacteria in real biofilms from the food industry and in experimental research of factors (nutritive, culturing, stimulating and inhibiting) influencing the adhesion of selected anaerobic food-spoiling species to solid surfaces. The project is based on comparison between adhesion intensities of experimental data and growth rates of anaerobic bacteria adhered to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (X/DLVO theory, balance of interfacial free energies) using the physicochemical properties of interacting surfaces as inputs. The results will help to clarify the role of anaerobes in biofilm formation, in industrial practice to decrease the risks of biofilm formation by modifying the environmental conditions and surface properties of solids, and in processes of surface cleaning and sanitation.

Surface interactions of miroalgae

This project will carry out experimental research of factors (nutritive, growth conditions) influencing adhesion of industrially promising microalgae (food, environmental processes) to solid surfaces. The project is based on comparison of experimental data on adhesion intensities of microalgae, grown under different conditions to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (DLVO theory, balance of interfacial free energies) using physicochemical properties of interacting surfaces. The results are applicable both in cases when suppression of algal adhesion is desired (inner wall of photobioreactors) and in processes (biodegradation, biotransformation) where algal biofilm formation (immobilization) is wanted.

Collaboration

Image galery

Lab AS55
Japonochytrium in reactor
Pophyridium cruentum
Photobioreactors
Airlift photobioreactor
Algal beer
Chlorella vulgaris

Updated: 13.10.2017 12:45, Author: Martin Halecký

Selected Results

Branyik T., Humhal T., Kastanek P. (2017) Cultivating sea protist biomass, preferably microorganisms of Thraustochytriales, by performing cultivation in cultivation medium comprising waste brine solution from process of demineralization of sweet whey. European Patent Number: EP2990474-A1 (on-line)

Baresova M., Pivokonsky M., Novotna K., Naceradska J., Branyik T.: An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Research 122, 70-77 (2017). ISSN: 0043-1354, IF 6,942. (on-line)

Humhal T., Kastanek P., Jezkova Z., Cadkova A., Kohoutkova J., Branyik T.: Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4. Bioprocess and Biosystem Engineering, 40, 395-402 (2017). ISSN: 1615-7591, IF 1,87. (on-line)

Šafařík I., Procházková G., Pospíšková K., Brányik T.: Magnetically modified microalgae and their applications. Critical Reviews in Biotechnology doi: 10.3109/07388551.2015.1064085, ISSN 0738-8551, IF = 7.178. (on-line)

Bittner M., de Souza A.C., Brozova M., Matoulkova D., Dias D.R., Branyik T.: Adhesion of anaerobic beer spoilage bacteria Megasphaera cerevisiae and Pectinatus frisingensis to stainless steel. LWT - Food Science and Technology 70, 148-154 (2016). ISSN 0023-6438, IF 2.416. (on-line)

Jelínek L., Procházková G., Quintelas C., Beldíková E., Brányik T.: Chlorella vulgaris biomass enriched by biosorption of polyphenols, Algal Research 10, 1-7 (2015). ISSN 2211-9264, IF 4.095. (online)

Procházková G., Kaštánek P., Brányik T.: Harvesting freshwater Chlorella vulgaris with flocculant derived from spent brewer’s yeast, Bioresource Technology 177, 28–33 (2015). ISSN 0960-8524, IF 5.039. (online)

Procházková G., Brányiková I., Zachleder V., Brányik T.: Effect of nutrient supply status on biomass composition of eukaryotic green microalgae. Journal of Applied Phycology 26, 1359–1377 (2014). ISSN 0921-8971, IF 2.492. (online)

Procházková G., Šafařík I., Brányik T.: Harvesting microalgae with microwave synthesized magnetic microparticles. Bioresource Technology 130, 472-477 (2013). ISSN 0960-8524, IF 5,039. (online)

×


UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
switch to desktop version