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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2017
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department

As the Department name indicates, the Department of Biotechnology is engaged in education, science, and consultancy activities in biotechnologies from the chemical, technical, and engineering points of view. The Department's core activities are in brewing, biotechnologies for environmental protection, alternative fuel production, issues of biofilms, and the production of biodetergents and enzymes.

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Our Department offers higher education in fields of traditional as well as modern biotechnologies, e.g. chemical and pharmaceutical industries and environmental protection, and bioengineering. The Department is accredited to provide three-year Bachelor programmes, two-year Master programmes, and Doctoral programmes.

studies

Interested students can participate in scientific and research work at the Department from the outset of their studies.

Given a wide range of contacts in Europe and worldwide, it is possible to apply for study or research fellowships.

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research

Research focuses on biotechnological processes in their entireties—including the behavior of biological agents; the study of unit operations which are involved in modern biotechnology, including downstream processing; aspects of bioengineering and the modeling and simulation of bioprocesses; the application of biotechnological processes in the food, pharmaceutical, biotechnology and chemical industries; and environmental protection.

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 partners

Collaboration with domestic and international industrial and research partners on a wide range of joint projects involving basic and applied research, scaling of technologies and reactors, solution of technological problems by form of grants or cooperation contracts is an essential part of our scientific and research work. An integral part of this cooperation is the involvement of students of all learning levels in such projects to improve their knowledge and skills.

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Explore individual group focus and collaboration potential:

aplikovaná_en (originál)bioing_en (originál)pivo_en (originál)mikrobiální procesy_en (originál)molekulára_en (originál)

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We are focused on a variety of topics, which have a common denominator: trying to understand the underlying mechanisms of biological activity of microorganisms and microbial communities. The main goal is to apply the combined knowledge of biotechnology and medicine to engineer inhibitors of microbial growth or finding new ways to inhibit biofilm formation, protect the environment or utilize new biologically active substances. Another topic of interest of our laboratory is nanoparticles: we study both their production by physicochemical or biological means and their antimicrobial activity. An inherent part of our work is cooperation with the industry, particularly wine industry, with a focus on plant waste valorisation. Our laboratory is well equipped for performing microbiological and biochemical techniques, as well as image analysis. We also have access to analytical equipment such as transmission electron microscope, confocal microscope or mass-spectrometer.

Research (originál)Teaching (originál)Significant results (originál)Students involment (originál)Lab-off time (originál)Collaboration and Projects (originál)

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Research and industrial partners

Research projects

Ecology of extremophilic microorganisms in Czech spring waters of cultural heritage significance, 18-00036S
Funding provider: Czech Science Foundation (GACR)
Researcher in Charge: Assoc. Prof. Ing. Ondřej Uhlík PhD, Department of Biochemistry and Microbiology, UCT Prague
Co-researcher: Assoc. Prof. Irena Kolouchová PhD, Department of Biotechnology, UCT Prague
Period: 2018-2020

Relationship between Vitis vinifera L. endophytes, and its metabolome, 18-26463S
Funding provider: Czech Science Foundation (GACR)
Researcher in Charge: Assoc. Prof. Ing. Milena Stránská PhD, Department of Food Analysis and Nutrition, UCT Prague
Co-researcher: Assoc. Prof. Irena Kolouchová PhD, Department of Biotechnology, UCT Prague
Period: 2018-2020

Wine products with added value, TH03030271
Technology Agency of the Czech Republic (TAČR) – Program Epsilon
Applicant: Assoc. Prof. Irena Kolouchová, Ph.D., Department of Biotechnology, UCT Prague
Period: 2018-2021

Cooperation on the development of wines with heightened resveratrol content, 17/005/16220/564/000088
The State Agricultural Intervention Fund (SZIF)
Applicant: Nové vinařství, a.s.
Co-applicant: Department of Biotechnology, UCT Prague, Assoc. Prof. Irena Kolouchová, Ph.D.,
Period: 2018-2021

Research and development of excipients for plant nutrition and vitality, TH04030109
Technology Agency of the Czech Republic (TAČR) – Program Epsilon
Applicant: Assoc. Prof. Irena Kolouchová, Ph.D., Department of Biotechnology, UCT Prague
Period: 2019-2022

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Christmas Party (2019)

International Conference on Chemical Technology in Mikulov (2019)

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Bachelor programme:

  • Biotechnology I
  • Physiology of Industrial Microorganisms

Master programme:

  • Applied Cell Biology
  • Biochemistry of Secondary Metabolites
  • Biotechnological Applications of Microorganisms
  • Biotechnology in chemical and pharmaceutical production
  • Environmental biotechnology
  • Viticulture, production of non-alkoholic and low-alcoholic beverages
  • Cultivation Techniques and Modeling of Bioprocesses
  • Subjects with Individual Assignments:
    • Biotechnology: Laboratory I, II
    • Pharmaceutical Biotechnology: Laboratory I, II, III
    • Biotechnology: Semestral Work
    • Biotechnology: Seminar and Specialised Project
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What you can learn in our lab

  • Basic microbiology techniques: cultivation and analysis of microorganisms
  • Working with microbial biofilms, their cultivation on various different materials (from polystyrene microtitre plates and titanium alloys used in medicine to packed columns and flow cell chambers) and the subsequent evaluation of biofilm formation or eradication under the influence of different antimicrobial agents
  • Cultivation of extremophilic microorganisms focusing on their biotechnologically relevant characteristics (e.g. lipid production)
  • Biosynthesis of nanoparticles (using plant extracts), their characterization and antimicrobial activity
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Paldrychová M., Vaňková E., Scholtz V., Julák J., Sembolová E., Maťátková O., Masák J.: Effect of non-thermal plasma on AHL-dependent QS systems and biofilm formation in Pseudomonas aeruginosa: Difference between non-hospital and clinical isolates, AIP Advances 9, 055117, 2019 (on-line)

Gharwalova, L., Sigler, K., Dolezalova J., Masak, J., Rezanka, T., Kolouchova, I.: Resveratrol suppresses ethanol stress in winery and bottom brewery yeast by affecting superoxide dismutase, lipid peroxidation and fatty acid profile. World Journal of Microbiology and Biotechnology. 2017 DOI: 10.1007/s11274-017-2371-x. IF 1,658. (on-line)

Matatkova, O., Kolouchova, I., Kvasnickova. E., Jezdik, R., Masak, J., Cejkova, A.: Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum. Chemical Papers. 71(8):1471-1480, 2017. IF 1,258. (on-line)

Pádrová K., Maťátková O., Šiková M., Füzik T., Masák J., Čejková A., Jirků V.: Mitigation of Fe0 nanoparticles toxicity to Trichosporon cutaneum by humic substances, New Biotechnology 33(1), 144-152, 2016 (on-line)

Kolouchova I., Sigler K., Schreiberova O., Masak J., Rezanka,T.: New yeast-based approaches in production of palmitoleic acid. Biosource technology 192, 726-734 (2015) ISSN: 0960-8524, IF = 4,494. (on-line)

Jirků V., Čejková A., Schreiberová O., Ježdík R., Masák J.: Multicomponent biosurfactants — A “ Green Toolbox” extension, Biotechnology Advances 33(6), 1272–1276 (2015) ISSN: 0734-9750, IF 8.905. (on-line)

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Biofilms

Researchers: Jan Masák, Prof., PhD, Alena Čejková, Prof., PhD, Olga Maťátková, Assoc. Prof., PhD, Irena Kolouchová, Assoc. Prof., PhD, Eva Vaňková, PhD

Biofilms can be defined as structured communities of microorganisms colonizing solid abiotic surfaces, tissues, or phase interfaces, surrounded by matrix (EPS, extracellular polymeric substances) and exhibiting various distinct cell phenotypes. It is one of the most common forms of life for microorganisms. The presence of microorganisms in biofilms is the result of purposeful behaviour regulated at the level of intercellular communication based on the production and perception of signalling molecules. Microorganisms living in a biofilm have a different phenotype when compared to planktonic populations. This phenotype is characterized by increased resistance to toxic substances and generally towards the external environment, high production of extracellular polymeric compounds and significantly altered protein profiles. There is also a change in catabolism and anabolism. The biofilm formation ability is also a manifestation of virulence of the pathogenic microorganisms. Based on these principles, we explore the following topics:

  • Biofilm preparation methodologies on different carriers and in different environmental conditions. Observing the properties of mature biofilms – metabolic activity, total biofilm biomass, EPS production, biofilm structure etc.
  • Study of the anti-biofilm activity of natural substances that interfere with the regulation of biofilm formation (interference with microbial signalling molecules) or affect the stability of biofilm matrix (biologically active substances: rhamnolipids, etc.). Utilization of this knowledge to suppress microbial colonization of instruments or implants used in medicine.
  • Study of virulence factor production in both planktonic and biofilm forming populations, searching for new methods of inhibition of virulence factor production.
  • Study of the relationships between the presence and concentration of specific signalling molecules (especially acyl-homoserine lactones) and formation of bacterial biofilms and their stability.
  • Study of metabolic pathways and increased resistance of biofilm populations enabling efficient decomposition of toxic substances in the environment.

3D representation of Trichosporon cutaneum biofilm created using confocal microscopy: control (a), 160 mg/L of resveratrol (b).

Interaction of Nanoparticles and Microorganisms

Researchers: Jan Masák, Prof., PhD, Alena Čejková, Prof., PhD, Olga Maťátková, Assoc. Prof., PhD

Due to their size, nanoparticles have physicochemical properties that differ from their respective macroscopic analogues. Particle sizes ranging from 1 to 100 nm enable a relatively easy penetration across cell membranes into the cytosol, where particles can further interact with cellular components. A high value of their specific surface area facilitates their interaction with the cell surface. A big advantage of nanoparticles lies in their complex mechanisms of action. It is clear that nanoparticles interfere with multiple cellular processes simultaneously; therefore, the probability of development of microorganisms resistant to the effects of nanoparticles is very limited. For biological applications, considerable attention is given to nanoparticles of metals and their oxides.

In the context of the comprehensive knowledge of biological effects of nanoparticles Fe0, Au0, Ag0 (and others) on single-celled organisms (prokaryotes and eukaryotes), we study the following topics:

  • Impact of nanoparticles on populations of single cell microorganisms monitored at the level of growth characteristics and reproductive activity.
  • Study of changes in cell morphology and cytology in connection with the toxicity of nanoparticles.
  • Investigation of oxidative stress induced by some nanoparticles, including lipid peroxidation, protein carbonylation, and the accumulation of reactive oxygen species activity of enzymes that protect cells from oxygen radicals.
  • Ability to protect cells from the toxic effects of nanoparticles by applying natural substances.

Ag nanoparticles formed via biosynthesis using extract from Vitis vinifera agriculture waste.

Microbial lipid production

Researchers: Irena Kolouchová, Assoc. Prof., PhD, Olga Maťátková, Assoc. Prof., PhD, in collaboration with Institute of Microbiology of the CAS, v. v. i.

Microorganisms produce lipids as storage compounds with triacylglycerols (TAGs) as their main constituents. Information about the exact composition of TAG mixtures is crucial for understanding their biosyntheses and their overproduction. The full characterization of the TAG fraction for an oil/fat requires the separation of all individual TAGs followed by the quantitation of separated species.

Many species of algae and cyanobacteria have the ability to produce substantial amounts (e.g., 20-50% of dry cell weight) of triacylglycerols as storage lipids. Cold- and hot-adapted microorganisms also have considerable potential in biotechnological applications because they accumulate large quantities of lipids and are able to regulate efficiently their lipid composition and adjust membrane fluidity.

Oleaginous bacteria that produce microbial oils in high quantities are able to accumulate TAGs under nitrogen starvation and are able to be cultivated on unusual carbon sources. Lipid hormones (lipokines) are fatty acids with many positive effects on human health. An important lipokine is palmitoleic acid (9-16:), which contributes to reduced inflammation, protection of the cardiovascular system, and inhibition of oncogenesis. Yeasts are attractive sources of palmitoleic and other beneficial fatty acids that are found in yeast cells either as polar lipids, i.e., phospholipids or glycolipids; or as neutral lipids, such as triacylglycerols.

  • Possibilities of microbial lipid production by yeasts, bacteria and algae
  • Potential production of essential fatty acids by microorganisms
  • Production of specific fatty acids for the pharmaceutical and cosmetics industries
  • Biodiesel production using yeast
  • Study of lipids from extremophile microorganisms

Vitis vinifera

doc. Ing. Irena Kolouchová, PhD., doc. Ing. Olga Maťátková, PhD., prof. Ing. Alena Čejková, CSc., spolupráce: EPS biotechnology s.r.o., Vinné sklepy Kutná Hora s.r.o., Vinselekt Michlovský a.s., Nové Vinařství a.s.

The grapevine (Vitis vinifera) is a important cultural crop, which has been domesticated as one of the first fruit crops. It is used both for direct consumption, for the production of raisins and for processing in the wine industry. Grapevine is a rich source of antioxidants and polyphenolic substances.

The physiological state of grapevines is influenced by many factors, including climatic conditions (biotic and abiotic stress) in a given year and by the vineyard treatment. All these factors have an impact on health of the plant, which also affects the grapes yield and the content of antioxidants. The stress response mechanisms of the plant include the synthesis and transport of antioxidants and production of secondary metabolites that are synthesized not only by the vine but also by its endophytic microflora. The physiological state of vine is also influenced by the use of spraying in viticulture, nowadays with more frequent use of natural products for the protection of the plant and the environment.

Wine production technology is one of the traditional biotechnologies that have been used since ancient times. Biotechnological approaches and possibilities are now changing, both in the terms of new developments in the grape processing technology or filtration, but also in terms of new yeast strains with improved properties, used for the must fermentation.

Food manufacturers have an increasing interest in the benefits stemming from the presence of various nutritional compounds in their products. These include polyphenol antioxidants, such as resveratrol. Bioavailability is a limiting factor in the uptake of polyphenol compounds, which often originates in their limited solubility in water solutions. Interesting sources of polyphenol antioxidants include wine. The legislative requirements for wine production are such, that the increase in the content of nutritionally beneficial substances can only by done by the means of modification of the technology production.

  • Development of new technologies of wine production with increased content of biologically active substances
  • Development of technologies for production of low-alcohol and non-alcoholic wine drinks with biologically active substances and antioxidants
  • Possibilities of using wine waste as a source of biologically active substances
  • New ways of vine protection in organic agriculture
  • New technologies of wine drinks production
  • Changes in the endophytic microflora population during the growth of vine during the year and in the response to stress
  • Biotechnologically interesting products of the endophytic fungi associated with grapevine

Spirits technology

doc. Ing. Irena Kolouchová, PhD., doc. Ing. Olga Maťátková, PhD.

The manufacture of fruit spirits belong to the traditional biotechnologies. Like wine making, fruit spirits have been produced since antiquity. At present, the view towards the traditional production is changing, in particular with regard to the use of pure yeast cultures for the fermentation. Correct fermentation technology and the subsequent distillation can significantly improve the quality of the resulting product, and even the production yield can be increased.

  • Influence of fermentation and distillation technology on analytical and sensory quality of spirits
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Lab team (originál)

The substantial part of our scientific research is devoted to the study of viruses. We investigate retroviruses, i.e. viruses that can reversely transcribe their genetic information from RNA to DNA, and we are focused mainly on one important retroviral member: HIV, a causative agent of AIDS. We are also interested in flaviviruses, especially their representatives such as Dengue virus, tick-borne encephalitis virus, and Zika virus. We study a certain stage in the replication cycle of these viruses, during which hundreds to thousands of copies of viral structural proteins and viral genomic information gather at some specific place within the infected cell, and based on their mutual interactions the assembly of new viral particle is initiated. In order to inhibit these steps and prevent thus the viral infection, our goal is to understand the structural-functional mechanisms of these protein-protein and protein-RNA interactions, which are critical for the formation of the new viral particle.

To fulfill our research, we use the equipment available at our department for DNA/RNA analysis, Protein analysis and Tissue culture.

GIF_500x500 (originál)

box Research (originál)box Teaching (originál)box significant results (originál)box students involvement (originál)LAB OFF time (originál)

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2019

Ivana Křížová placed sixth in the second round of the University Fencing League.

sc_PB167661v (originál)

2018

Our fresh graduate students

Naše letošní slečny inženýrky (originál)

Spring welcome party under sakura trees

Vítání jara pod sakurama (originál)

Inevitable cleaning

Nezbytný úklid (originál)

Lukáš Pekárek won the Czech national final of FameLab!

Lukáš Pekárek- zvítězil v celonárodním kole FameLab (originál)

Lukáš Pekárek- zvítězil v celonárodním kole FameLab2 (originál)

Appbiotics

Appbiotics3 (originál)A team of Lukáš Pekárek and Filip Kaufman created a new application “Appbiotics” for the Android mobile platform, which is used to fight the antibiotic resistance. Their effort was awarded by the second place in the competition Angelini University Award 2017/2018. https://www.appbiotics.cz

Appbiotics1 (originál)

Appbiotics2 (originál)

An exceptionally good farewell party for Lorenzo

Vyvedená rozlučka s Lorenzem1 (originál)

Vyvedená rozlučka s Lorenzem2 (originál)

Our team Viruses busters fought bravely with a very tricky “Run competition” around the UCT buildings

2018- Náš tým Krotitelé virů (originál)

2017

Our team in 2017

2017 (originál)

The sweetest award

to nejsladší oceněn (originál)

Our team Viruses busters first in the action

Náš tým Krotitelé virů poprvé v akc (originál)

Nitrogen cooled ice cream…………… and what else?

není nad dusíkem (originál)

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Retroviruses (originál)Box Flaviviruses (originál)

Box interaction of viral and cellular proteins (originál)Box study of selected human proteins (originál) ◳ coronaviruses_box (jpg) → (originál)

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Our significant results and achievements

Ing. Alžběta Dostálková, Ph.D. has won the Doctorandus Award for Natural Sciences of Veolia in the most prestigious Czech award Czech Head 2022 for the study of interactions leading to the folding of retroviral particles. 

 ◳ česká hlava Alžběta (jpg) → (originál)

Ing. Filip Kaufman placed second in the conference competition International Graduate Symposium on Industrial Biotechnology (Jiangnan University, Wuxi, China) with poster: "Combined approach to identification of novel inhibitors of HIV-1 capsid protein".

FK1 (originál)

FK2 (originál)

Michaela Rumlová, PhD associate professor: Grant agency of Czech Republic Award in 2017

(video in Czech)

play

Lukáš Pekárek: Ministry of Education, Youth and Sport Award in 2018

Lukáš Pekárek- Cena Ministra školství a tělesné výchovy ČR 2018 (originál)

Our significant papers:

Bharat,T. A.M.; Davey,N. E.; Ulbrich,P.; Riches,J. D.; de Marco,A; Rumlova,M.; Sachse,C.; Ruml,T.; Briggs,J. A.G. (2012): Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy. Nature, 487(7407):385-9

Schur F., Hagen W., Rumlova M., Ruml T., Müller B., Kraeusslich H.-G, Briggs J.,.: The structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution. Nature (2015), 517, 505-508

Hadravová R., Rumlová M., Ruml T.: FAITH – Fast Assembly Inhibitor Test for HIV. Virology (2015) 486, 78-87

Rumlová M. & Ruml T.: In vitro methods for testing antiviral drugs. Biotechnology Advances. 2018, 36, 3, 557-576.

Dostalkova, A.; Kaufman, F.; Krizova, I.; Kultova, A.; Strohalmova, K.; Hadravova, R.; Ruml, T.; Rumlova, M.: Mutations in the Basic Region of the Mason-Pfizer Monkey Virus Nucleocapsid Protein Affect Reverse Transcription, Genomic RNA Packaging, and the Virus Assembly Site. J.Virol, 2018, 92, 10

Qu K., Glass Bärbel., Doležal M., Schur F., Murciano B, Rein A., Rumlová M., Ruml T., Kräusslich H-G., Briggs JAG. Structure and architecture of immature and mature murine leukemia virus capsids: PNAS 2018, 115, 50, E11751-E11760

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What can we teach you:

  • Basic techniques of molecular biology: DNA, RNA isolation, cloning, mutagenesis, expression of recombinant proteins in bacterial or tissue cells and their identification, isolation and purification, methods for monitoring and quantification of protein-protein, protein – DNA and protein – RNA interactions, basic virology techniques: preparation of proviral vectors, quantification of viral stock solutions
  • determination and quantification of virus production in tissue cells by immunochemical methods: ELISA and western blot, determination of virus infectivity by flow cytometry, basic techniques of tissue culture cell cultivation, cell-based methods: viability, the progression of the life cycle, apoptosis, necrosis, oxidative stress….

Zapojení studentů 1 (originál)

Zapojení studentů 2 (originál)

Zapojení studentů 3 (originál)

Zapojení studentů 4 (originál)

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Winter semester: Molecular biology, syllabus here.

syllabus Ang (originál)

Cultivation techniques, laboratory methods:

  • Techniques of molecular biology and their application in  biotechnology
  • Expression, isolation and purification of recombinant proteins
  • Determination of cell cycle using flow cytometry
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Our laboratory engages with engineering and biological aspects of biotechnologies, especially in the area of processes optimization (e.g. media composition, culture conditions) and mathematical modeling of bioprocesses. The main research topics include:

[ikona] => [obrazek] => lom_vertical.jpg [obsah] =>
  • Harnessing the biosorption potential of phototrophic microorganisms (study of mechanism and quantification of sorption of organic molecules)
  • Biotechnology of marine organisms (cultivation processes, utilization of waste water)
  • Study of the mechanism of adhesion of microbial contaminants of food to solid substrates
  • Biotechnology of microalgae (culture techniques, utilization of waste water, new methods of harvesting algae, adhesion of algae to surfaces)

Research group members

Main research topics

Biosorption potential of phototrophic microorganisms

 Microalgal cell surfaces display a variety of binding sites, which allow biosorption of biologically active compounds. However, little is known about biosorption potential of phototrophic microorganisms towards organic molecules. The project aims to clarify fundamental relationships between adsorbent composition (microalgae and its fractions) and chemical structure of adsorbate. The scope of experimental work is defined by phototrophic taxons (Chlorella, Porphyridium, Arthrospira) and series of target compounds (polyphenols) with varying structures. Data on biosorption of individual polyphenols and their mixtures will be supported by physicochemical and chemical characterization of interacting entities, rheological data on dispersed systems and mathematical description of reaction equilibrium and kinetics. Based on this information, experiments will be performed to test 1) biosorption of ecologically, nutritionally, therapeutically relevant compounds of microalgal biomass or its fractions, 2) effective separation methods of dispersed sorbents from solutions, and 3) scale-up approaches.

Bioactive compounds from marine microorganisms

Marine unicellular microorganisms are very rich in bioactive compounds and thus may be used in several biological applications. The most studied substances are polyunsaturated fatty acids (PUFAs), sterols, proteins and enzymes, and vitamins and pigments, which find applications in areas such as human and animal nutrition, therapeutics, and aquaculture. This project will focus mainly on marine microorganisms (e.g. algae, cyanobacteria, bacteria), their cultivation and culture condition-induced changes in the composition of biomass, and the identification of bioactive compounds with biotechnological potential.

Anaerobic food spoiling bacteria and their biofilm forming potential

The project is aimed at isolation and identification of anaerobic bacteria in real biofilms from the food industry and in experimental research of factors (nutritive, culturing, stimulating and inhibiting) influencing the adhesion of selected anaerobic food-spoiling species to solid surfaces. The project is based on comparison between adhesion intensities of experimental data and growth rates of anaerobic bacteria adhered to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (X/DLVO theory, balance of interfacial free energies) using the physicochemical properties of interacting surfaces as inputs. The results will help to clarify the role of anaerobes in biofilm formation, in industrial practice to decrease the risks of biofilm formation by modifying the environmental conditions and surface properties of solids, and in processes of surface cleaning and sanitation.

Surface interactions of miroalgae

This project will carry out experimental research of factors (nutritive, growth conditions) influencing adhesion of industrially promising microalgae (food, environmental processes) to solid surfaces. The project is based on comparison of experimental data on adhesion intensities of microalgae, grown under different conditions to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (DLVO theory, balance of interfacial free energies) using physicochemical properties of interacting surfaces. The results are applicable both in cases when suppression of algal adhesion is desired (inner wall of photobioreactors) and in processes (biodegradation, biotransformation) where algal biofilm formation (immobilization) is wanted.

Collaboration

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Branyik T., Humhal T., Kastanek P. (2017) Cultivating sea protist biomass, preferably microorganisms of Thraustochytriales, by performing cultivation in cultivation medium comprising waste brine solution from process of demineralization of sweet whey. European Patent Number: EP2990474-A1 (on-line)

Baresova M., Pivokonsky M., Novotna K., Naceradska J., Branyik T.: An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Research 122, 70-77 (2017). ISSN: 0043-1354, IF 6,942. (on-line)

Humhal T., Kastanek P., Jezkova Z., Cadkova A., Kohoutkova J., Branyik T.: Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4. Bioprocess and Biosystem Engineering, 40, 395-402 (2017). ISSN: 1615-7591, IF 1,87. (on-line)

Šafařík I., Procházková G., Pospíšková K., Brányik T.: Magnetically modified microalgae and their applications. Critical Reviews in Biotechnology doi: 10.3109/07388551.2015.1064085, ISSN 0738-8551, IF = 7.178. (on-line)

Bittner M., de Souza A.C., Brozova M., Matoulkova D., Dias D.R., Branyik T.: Adhesion of anaerobic beer spoilage bacteria Megasphaera cerevisiae and Pectinatus frisingensis to stainless steel. LWT - Food Science and Technology 70, 148-154 (2016). ISSN 0023-6438, IF 2.416. (on-line)

Jelínek L., Procházková G., Quintelas C., Beldíková E., Brányik T.: Chlorella vulgaris biomass enriched by biosorption of polyphenols, Algal Research 10, 1-7 (2015). ISSN 2211-9264, IF 4.095. (online)

Procházková G., Kaštánek P., Brányik T.: Harvesting freshwater Chlorella vulgaris with flocculant derived from spent brewer’s yeast, Bioresource Technology 177, 28–33 (2015). ISSN 0960-8524, IF 5.039. (online)

Procházková G., Brányiková I., Zachleder V., Brányik T.: Effect of nutrient supply status on biomass composition of eukaryotic green microalgae. Journal of Applied Phycology 26, 1359–1377 (2014). ISSN 0921-8971, IF 2.492. (online)

Procházková G., Šafařík I., Brányik T.: Harvesting microalgae with microwave synthesized magnetic microparticles. Bioresource Technology 130, 472-477 (2013). ISSN 0960-8524, IF 5,039. (online)

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Laboratoř bioinženýrství (originál)

In Laboratory of Bioengineering we deal with production and decontamination biotechnologies from the point of view of bioreactors and control of processes as well as from the point of view of (micro)biology, biochemistry and genetics. Using submerged and biofilm photobioreactors we study the application of unicellular marine and freshwater algae and photosynthesizing bacteria to produce biologically active substances. We use modern technologies of multispectral analysis and 3D scanning to study the potential of microorganisms and their products to produce biopreparations e.g. biostimulants and biopesticides for plant treatment in sustainable agriculture. We also focus on enzyme synthesis and recycling of sustainable polyurethane materials with maximum use of natural and renewable raw materials using modern concepts such as "Green chemistry", "Cradle-to-grave" and "Cradle-to-cradle". Using contact angle and zeta potential we study the surface properties of both biomass and packing materials, which we further use in the study of biofilm formation, flocculation, flotation and emulsification.

To fulfill our research, we use bioreactors and other equipment available at our department for cell cultivation, downstream processesHPLC and GC analyses and special methods.

lll2 (originál)

veda_en (originál)pedagogika_en (originál)vysledky_en (originál)zapojení studentu_en (originál)lab-off_en (originál)spolupráce_en (originál)

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We cooperate with a number of Czech and foreign companies and universities, where our students can apply and mainly expand their knowledge and skills:

Our research is supported not only by enterprises but also by research projects:

Biofiltration impregnated composite materials and substrates, FW01010370
Funding provider: Technology Agency of the Czech Republic (TACR) – programme TREND
Researcher in Charge: Ing. Luboš Zápotocký Ph.D. (Dekonta a.s.)
Co-researcher: Assoc. Prof. Martin Halecký PhD, Department of Biotechnology, UCT Prague
Period: 2020-2023

Use of wastes from dairies for production of new dairy products and dietary supplements with the addition of microalgae and their components, QK1910300
Funding provider: Ministry of Agriculture of the Czech Republic
Researcher in Charge: Prof. Tomáš Brányik PhD, Department of Biotechnology, UCT Prague
Participants: EcoFuel s.r.o., Ekomilk a.s., Ingredia s.r.o., VÚM s.r.o.
Period: 2019-2022

Microporous polyurethanes with adjustable rate of biodegradation, 19-08549S
Funding provider: Czech Science Foundation (GACR)
Researcher in Charge: Ing. Hynek Beneš PhD, DSc, Institute of Macromolecular Chemistry, Czech Academy of Sciences
Co-researcher: Assoc. Prof. Martin Halecký PhD, Department of Biotechnology, UCT Prague
Period: 2019-2021

Influence of algal organic matter on properties of flocs formed during coagulation/flocculation in water treatment, GAČR 18-05007S
Funding provider: Czech Science Foundation (GACR)
Researcher in charge: doc. RNDr. Martin Pivokonský Ph.D., Institute of Hydrodynamics of the Czech Academy of Sciences, Prague
Co-researcher: prof. Tomáš Brányik Ph.D., Department of Biotechnology, UCT Prague
Period: 2018-2020
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Zuzka in Colombia at Universidad de San Buenaventura Cartagena (2020)

Kolumbie - Zuzana Ježková 1_w (originál)

Kolumbie - Zuzana Ježková 2_w (originál)

At the UCT's cottage in Krkonoše Mountains (2020) - On the Road

Pec pod Sněžkou 2020_Raseliniste (originál)

At the UCT's cottage in Krkonoše Mountains (2020) - Meal at the cottage - dron view

Pec pod Sněžkou 2020_dronfee (originál)

Festival of Science (2019)

Festival vědy 2019_w (originál)

Honza Caught Fish on Internship at Partner University of North Dakota, USA (2019)

Foto9_2019_w (originál)
“Lesamáj” in the Dejvice Campus (2018)

Lesamaj_2018 (originál)
Krkonoše Mountains (2018)

Krkonoše 2018 (originál)
Krkonoše Mountains - Conquest of Sněžka mountain (2018)

Snezka_2018_w (originál)
Honza and Maryna's Wedding - Group Photo (2018)

Chalupovi (317 of 760)_w (originál)
Honza and Maryna's Wedding - (Research) Groups Battle (2018)

Chalupovi (319 of 760)_w (originál)
Krkonoše Mountains (2017)

Krkonose 2017_w (originál)
Honza Won on Dragon Boats (2013)

Honza Draci lode_2013_w (originál)

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Functional materials for (bio)technologies

In collaboration with our research partners, we develop and test packing materials with various functional potentials combining specific surface properties e.g. specific surface area, hydrophobicity, pore size and distribution, sorption properties, mineral nutrient content, utilizable carbon and nitrogen content and inducers for microbial production of specific substances e.g. biopesticides. We test intentionally synthesized polyurethane foams with controlled biodegradability as packing materials for production and decontamination bioreactors (collaboration with Institute of Macromolecular Chemistry, Czech Academy of Sciences) and various types of carbonaceous materials e.g. biochars (collaboration with ENERGO Zlatá Olešnice and Institute of Energy of ICT Prague). We predict the suitability of materials as a biofilm carrier (X/DLVO theory and balance of interfacial free energy) and observe real surface colonization (microscopy and image analysis).

funkční materiály (originál)

Bioactive compounds from marine microorganisms

Marine unicellular microorganisms are very rich in bioactive compounds and thus may be used in several biological applications. The most studied substances are polyunsaturated fatty acids (PUFAs), sterols, proteins and enzymes, and vitamins and pigments, which find applications in areas such as human and animal nutrition, therapeutics, and aquaculture. This project will focus mainly on marine microorganisms (e.g. algae, cyanobacteria, bacteria), their cultivation and culture condition-induced changes in the composition of biomass, and the identification of bioactive compounds with biotechnological potential.

Biologicky aktivní látky z mořských_w (originál)

Surface interactions of microalgae

This project will carry out experimental research of factors (nutritive, growth conditions) influencing adhesion of industrially promising microalgae (food, environmental processes) to solid surfaces. The project is based on comparison of experimental data on adhesion intensities of microalgae, grown under different conditions to solid materials (glass, plastics, steel etc.), with predictions of adhesion made according to mathematical models (DLVO theory, balance of interfacial free energies) using physicochemical properties of interacting surfaces. The results are applicable both in cases when suppression of algal adhesion is desired (inner wall of photobioreactors) and in processes (biodegradation, biotransformation) where algal biofilm formation (immobilization) is wanted.

Povrchové interakce jednobuněčných řas - DVLO (originál)

Biopesticides

Biopesticides are a key response to increased environmental quality as well as food quality requirements. In cooperation with research partners we deal with chitinase-based biopesticides (colaboration with Dekonta a.s.) and Pythium oligandrum (colaboration with Biopreparáty, s.r.o.). Chitinase-based biopesticides can be used as insecticides and fungicides. We focus on optimizing production process using biofilm systems on specifically synthesized polyurethane foam carriers with controlled biodegradability. Pythium oligandrum can be used in medicine, as a biopesticide in agriculture, and as a prevention of mold development in the entire malt production chain in the food industry (prevention of mycotoxins contamination).

biopesticidy_w (originál)

Technologies and (bio)reactors for wastewater and gas treatment

We are engaged in the use of packed bed and free-cell bioreactors for removal of volatile organic compounds (VOCs - petrol, diesel, mixtures of acetone and styrene and paint solvents and odorous substances) from waste gases and micropollutants (pharmaceuticals and pesticides) from contaminated water. In particular, we study the synergistic effects of the combination of physical-chemical and biological approaches such as multiphase bioreactors (absorption-biodegradation) and the combination of adsorption pre-columns with bioreactor or adsorption packing materials (adsorption - biodegradation).

biofiltry (originál)

Biosorption potential of phototrophic microorganisms

 Microalgal cell surfaces display a variety of binding sites, which allow biosorption of biologically active compounds. However, little is known about biosorption potential of phototrophic microorganisms towards organic molecules. The project aims to clarify fundamental relationships between adsorbent composition (microalgae and its fractions) and chemical structure of adsorbate. The scope of experimental work is defined by phototrophic taxons (Chlorella, Porphyridium, Arthrospira) and series of target compounds (polyphenols) with varying structures. Data on biosorption of individual polyphenols and their mixtures will be supported by physicochemical and chemical characterization of interacting entities, rheological data on dispersed systems and mathematical description of reaction equilibrium and kinetics. Based on this information, experiments will be performed to test 1) biosorption of ecologically, nutritionally, therapeutically relevant compounds of microalgal biomass or its fractions, 2) effective separation methods of dispersed sorbents from solutions, and 3) scale-up approaches.

Biolosorbce (originál)

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Potočár T., Pereira J.A.V., Brányiková I., Barešová M., Pivokonský M., Brányik T.: Alkaline flocculation of Microcystis aeruginosa induced by calcium and magnesium precipitates. Journal of Applied Phycology, 32, 329–337(2020) IF 2,635 (on-line)

Halecký M., Kozliak E.: Modern Bioremediation Approaches: Use of Biosurfactants, Emulsifiers, Enzymes, Biopesticides, GMOs, in Advanced Nano-Bio Technologies for Water and Soil Treatment, Chapter: Biotechnologies for Soil Treatment, 2020, Springer International Publishing, Basel, Switzerland ISBN 978-3-030-29839-5, DOI: 10.1007/978-3-030-29840-1 (on-line)

Chalupa J., Pocik O., Halecky M., Kozliak E.: Thermophilic waste air treatment of an airborne ethyl acetate/toluene mixture in a bubble column reactor: Stability towards temperature changes, Journal of Hazardous Materials, Journal of Hazardous Materials 384, (2020) IF 7,65; Q1 (on-line)

Branyikova I., Filipenska M., Urbanova K., Ruzicka M. C., Pivokonsky M., Branyik T.: Physicochemical approach to alkaline flocculation of Chlorella vulgaris induced by calcium phosphate precipitates. Colloids and Surfaces B: Biointerfaces 166, 54-60 (2018), IF 3,997 (on-line)

Jan Chalupa - Best Student Poster (2017)

Honza_poster_w (originál)

cena Jan Chalupa_web (originál)

Patent

Branyik T., Humhal T., Kastanek P. (2017) Cultivating sea protist biomass, preferably microorganisms of Thraustochytriales, by performing cultivation in cultivation medium comprising waste brine solution from process of demineralization of sweet whey. European Patent Number: EP2990474-A1 (on-line)

Patent EP2990474A1 (originál)

Baresova M., Pivokonsky M., Novotna K., Naceradska J., Branyik T.: An application of cellular organic matter to coagulation of cyanobacterial cells (Merismopedia tenuissima). Water Research 122, 70-77 (2017). ISSN: 0043-1354, IF 6,942. (on-line)

Vanek T., Silva A., Halecky M., Paca J., Ruzickova I., Kozliak E., Jones K.: Biodegradation of airborne acetone/styrene mixtures in a bubble column reactor. J. Environ. Sci. Health Part A-Toxic/Hazard. Subst. Environ. Eng. 52(9):905-915 (2017) (on-line)

Award Visionaries 2016 - Tomáš Brányik a Tomáš Humhal (2016)

Vizionáři 2016 (originál)

Šafařík I., Procházková G., Pospíšková K., Brányik T.: Magnetically modified microalgae and their applications. Critical Reviews in Biotechnology 36(5), IF = 7,178. (2016) (on-line)

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What you can experience with us and what can learn:

  • practical experimental work with different kinds of (bio)reactors
  • measurement and control of (bio)processes
  • cultivation techniques of mesophilic and thermophilic bacteria as well as yeasts, molds and freshwater and marine unicellular algae and photosynthetic bacteria
  • practical experimental work with HPLC and GC systems
  • determination of enzyme activity, advanced cell staining, biofilm formation kinetics (image analysis) and other practical microbiological and biochemical methods
  • surface characterization techniques including processing and interpretation of results using (X)DVLO theory
  • design and analysis of experiments and balancing and mathematical description of (bio)processes

    Youngest chemists

Malí chemici (originál)

3D chromatogram of the determination of micropolutants (pesticides) in water

pesticidy (originál)

Trickling biofilter for waste air treatment

skrápěný reaktor (originál)

Chlorella algae cultivation in photobioreactors

optimalizace_vertical (originál)

Fusarium solani on Rose Bengal agar

INTSA10_9 (originál)

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We are Involved in Teaching of Bioengineering and Biotechnology subjects:

  • Bioengineering I (in Czech)
  • Bioengineering II (in Czech)
  • Bioengineering (in English)
  • Basics of Bioengineering (in Czech)
  • Processes and Operations in Brewing Production (in Czech)
  • Trends in Biotechnologies (in English)
  • Cultivation Techniques and Bioprocess Modeling (in Czech)
    • Simultaneous degradation of acetone and styrene in a continuously operating submerged reactor (Laboratory task)
    • Heterotrophic Algae Cultivation (Laboratory Task)
    • Bioprocess Modeling (lecture)
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Our laboratory deals with education, research, and development in the fields of brewing and malting. Research activities investigate:

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  • Health-promoting compounds in brewing raw materials (hops, barley and beer
  • Microbiological, colloidal, and sensorial stability of beer
  • Microbial contaminants in beer and raw materials
  • Authentication of beer and brewing raw materials
  • Study of the properties of brewing yeast
  • Formation, stability, and decomposition of beer foam
  • Development of new products
  • Development of new analytical methods

  

Research group members

 

Main Research Topics

Increasing the Shelf Life of Unfiltered Beers

A consequence of increasing the production of unfiltered beer is the need for a significant extension of their shelf lives. For this purpose, we test the use of high hydrostatic pressure in plastic barrier packages. Moreover, we are also investigating the development and subsequent application of modular flow minipasterizeur for use in micro- and mini-breweries.

Increasing the Colloidal Stability of Czech Beers

Czech beers have higher polyphenol content and polypeptides than many other beers, and thus they tend to form colloidal haze. Colloidal stabilization is typically used for the sorption of synthetic stabilizers to extend shelf lives. We have developed a new generation of adsorbents capable of removing one of the precursors to colloidal haze. For this purpose, waste materials from the production of hop extracts and pellets are also being tested, which combines the advantages of increasing colloidal stability and preserving the antioxidant effects of beer.

Development of Bottom Yeast Strains with Improved Technological Properties

As a result of an expression of the cell wall, yeast with inserted genes produces anchored proteins with two functions. The first is a higher affinity towards polyphenolic compounds, where a microorganism may serve as an agent for increasing the colloidal stability of beer. The second protein is an enzyme catalysing the conversion of 2-acetolactate to acetoin. Accelerating this reaction would greatly simplify the process of maturing and reduce the risks associated with excessive levels of diacetyl, which causes a buttery flavour in beer. This research is conducted in collaboration with the Department of Biochemistry and Microbiology.

Biologically Active Substances of Hops

Used in folk medicine for several thousand years, hops contain substances with various beneficial health effects. These substances, which are also found in beer, can be isolated from hops and used in food supplements and final-stage drugs. Currently, we are testing methods related to the isolation and biotransformation of prenylated flavonoids (in particular, xanthohumol and 8-prenylnaringenin), including their strong phytoestrogenic properties that should facilitate their application in the suppression of menopausal symptoms.

Study of Beer Foam and Prediction of Gushing and the Possibilities of Its Suppression

Beer foam and beer foam stability are important organoleptic characteristics associate with beer but with a significant negative effect: gushing. Research into gushing includes methods for studying the causes and intensity of this phenomenon and the study of hydrophobins, primary triggers of gushing. We have developed a pressure column that enables our research of this complex phenomenon. We aim to develop a better understanding of the fundamental mechanisms of gushing and create proposals for its suppression (applying parasitic fungi to suppress the contamination of microflora in malt, which forms hydrophobins).

Study of Microbial Contamination in Breweries

The most widespread forms of contamination in breweries involve a microbial consortium form of biofilms. The scope of the project is to identify anaerobic beer spoilage bacteria (particularly from the Pectinatus and Megasphaera families) and experimentally study how they adhere to their chosen representatives on solid surfaces. By understanding the adhesion mechanism used by these species, we hope to develop new methods for suppressing adhesion or for increasing the effectiveness of the cleaning of surfaces in food processing plants.

Development of New Analytical Methods and Their Validation

Each beer is unique, and, because of this, we can analyse the composition and content of individual volatile compounds that characterize the origin of the raw materials used to produce it, its age, and immediate sensory conditions. We can even predict changes to a beer over time. New methods for conducting such analysis are being developed, mainly based on solid-phase microextension (SPME-GC-MS) for the determination of higher alcohols, esters and acids, and terpene compounds. Newly developed methods are then used to solve other research topics for the entire laboratory.

Use of Innovative Microorganisms for the Production of Beer

Increasing market competition has led breweries to expand the assortment of beers they offer. One promising possibility for the development of new products is the use of innovative microorganisms during the fermentation process. One such group of microorganisms is probiotics, which are beneficial to human health, improving immunity, and maintaining a balance of intestinal microflora. The aim of this research is to obtain a set of microorganisms with probiotic properties that are able to ferment brewing wort and/or to tolerate an environment of traditional and non-alcoholic beer without reducing viability. We are also studying the fermentation capacity of selected microorganisms, the formation of flavour compounds, and the effect of the presence of microorganisms on non-traditional beer flavour and colloidal stability.

Collaboration

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Prusova N., Dzuman Z., Jelinek L., Karabin M., Hajslova J., Rychlik M., Stranska M.: Free and conjugated Alternaria and Fusarium mycotoxins during Pilsner malt production and double-mash brewing, Food Chemistry, 369, 130926, doi.org/10.1016/j.foodchem.2021.130926, (2022) IF 7,514 (on-line)

Karaoglan S.Y., Jung R., Gauthier M., Kincl T., Dostálek P.: Maltose-Negative Yeast in Non-Alcoholic and Low-Alcoholic Beer Production. Fermentation 8(6), 273, (2022), IF 5,123 (on-line)

Karabín M., Haimannová T., Fialová K., Jelínek L., Dostálek P.: Preparation of Hop Estrogen-Active Material for Production of Food Supplements. Molecules 26(19), 6065 (2021) IF 4,411 (on-line)

Kinčl T., Dostálek P., Brányik T., Olšovská J.: High-gravity brewing without adjuncts - The effect on beer parameters. LWT-Food Science and Technology 148, 111755 (2021) IF 4,952 (on-line)

Štulíková K., Bulíř T., Nešpor J., Jelínek L., Karabín M., Dostálek P.: Application of High-Pressure Processing to Assure the Storage Stability of Unfiltered Lager Beer, Molecules, 25, 2414, (2020), IF 3,267 (on-line)

Nešpor J., Karabín M., Štulíková K., Dostálek P.: An HS-SPME-GC-MS Method for Profiling Volatile Compounds as Related to Technology Used in Cider Production, Molecules, 24, 2117, (2019), IF 3,06 (on-line)

Nešpor J., Andrés-Iglesias C., Karabín M., Montero O., Blanco C. A., Dostalek P.: Volatile compounds profiling in Czech and Spanish lager beers in relation to used production technology, Food Analytical Methods, 12(10), 2293-2305, (2019), IF 3,413 (on-line)

Postulkova M., Rezanina J., Fiala J., Ruzicka M.C., Dostalek P., Branyik T.: Suppression of fungal contamination by Pythium oligandrum during malting of barley. Journal of the Institute of Brewing 124, 336-340 (2018), IF 0,868 (on-line)

Nešpor J., Karabín M., Hanko V., Dostálek P.: Application of response surface design to optimise the chromatographic analysis of volatile compounds in beer. Journal of the Institute of Brewing 124 (3),  244-253 (2018), IF 0,868 (on-line)

Karabín M., Hanko V., Nešpor J., Jelínek L., Dostálek P.: Hop tannin extract: A promising tool for acceleration of lautering. Journal of the Institute of Brewing  (2018), 124, 374-380, IF 0,868 (on-line)

Carvalho D.O., Paulu A., Dostálek P., Guido L.F.: Measurement of catechin-7-O-glucoside from barley to malt. Journal of the Institute of Brewing (2018), 124, 359-364, IF 0,868 (on-line)

Krausová I., Mizera J., Dostále, P., Řanda Z.: Non-destructive determination of nitrogen in malting barleys by instrumental photon activation analysis and its comparison with the Dumas method. Journal of the Institute of Brewing 124 (1),  4-8 (2018), IF 0,868 (on-line)

Štulíková K., Karabín M., Nešpor J., Dostálek P.: Therapeutic perspectives of 8-prenylnaringenin, a potent phytoestrogen from hops. Molecules 23 (3), 660, 1-13 (2018), IF 3,098 (on-line)

Karabín M., Jelínek L., Kotrba P., Cejnar R., Dostálek P.: Enhancing the performance of brewing yeasts.  Biotechnology Advances 36 (3) 691-706 (2018),  IF 11,452 (on-line)

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The main topics:

  • Aerobic and anaerobic cultivation of bacteria, yeasts, and filamentous fungi
  • Production of primary and secondary metabolites
  • Characterization of microbial populations by use of fluorescent techniques
  • Molecular biology of solventogenic clostridia
  • Production of secondary metabolites by the fungus Monascus

Staff

 

Main research topics

Production of biofuels and bioplastics

Biotechnological production of biofuels (ethanol and butanol) and bioplastics precursors (succinic acid, lactic acid) by using first and second generation feedstocks. Design of fermentation process, selection of suitable feedstock, cultivation conditions and process arrangements (batch, fed-batch, continuous) for maximum yield and process productivity.  

Molecular biology of solventogenic clostridia

Analysis of gene function and comparative genome studies focused on the basic research of the organic solvents biosynthesis during acetone–butanol–ethanol (ABE) fermentation. Methods for genetic manipulation of solventogenic clostridia development, qPCR based gene expression monitoring and description of the biobutanol biosynthesis on the molecular level. Endospore formation research of Clostridium bacteria.

Analysis of microbial populations on the single cell level

Detailed analysis of microbial populations using fluorescent microscopy, flow cytometry, and fluorimetry. Analysis of cell viability, growth rate, enzyme activities, concentration of intracellular compounds (DNA, NA, proteins, PHA, GFP, …) and other cell functions using selective staining with fluorescent probes. Measurement of these parameters for bacterial and yeast populations within the cultivation process under the influence of stress factors and antimicrobial agents.

Production of recombinant proteins by microbial host strains

Use of continuous processed data for the design of fed-batch processes for high cell density cultivation for production of recombinant proteins. Analysis of online and offline variables, design of suitable process arrangement.

Processing of waste materials from agriculture and industry into value-added products

Physico-chemical and/or enzymatic processing of lignocellulosic materials (wheat, wood, etc.) and keratin rich material (e.g. chicken feathers) and use of substrates based on nutrients derived from these feedstocks for biotechnological production of value-added products, e.g. biofuels (bioethanol, biobutanol), precursors of bioplastics (lactic and succinic acids), and others.

Production of secondary metabolites

Production of oligoketide pigments by Monascus purpureus by solid state or submersed cultivation. Optimization of cultivation conditions for preferential biosynthesis of yellow pigments (monascin and ankaflavin). Isolation and determination of pigments, citrinin, and monacolin K. Testing the effects of yellow pigments on the rat metabolic syndrome (in cooperation with ICEM).  

Cooperation

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Patáková P. et al., Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level, Scientific Reports 9, 1371, 2019 (on-line)

Sedlar K. et al., Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq. BMC Genomics 19 (1), 415, 2018 (online)

Branska B. et al., Flow cytometry analysis of Clostridium beijerinckii NRRL B-598 populations exhibiting different phenotypes induced by changes in cultivation conditions. Biotechnology for Biofuels 11:99, 2018 (online)

Patakova P., Kolek J., Sedlář K., Koscova P., Branska B., Kupkova K., Paulova P., Provazník I.: Comparative analysis of high butanol tolerance and production in clostridia, Biotechnology Advances 36(3),721-738, 2018 (online)

Sedlar K., Kolek J., Provaznik I., Patakova P.: Reclassification of non-type strain Clostridium pasteurianum NRRL B-598 as Clostridium beijerinckii NRRL B-598. Journal of Biotechnology 244(20), 1-3 (2017) (online)

Kolek J., Sedlar K., Provaznik I., Patakova P. Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation and sonoporation. Biotechnology for Biofuels (2016) 9:14.DOI 10.1186/s13068-016-0436-y, (open access)

Paulová L., Patáková P., Branská B., Rychtera M., Melzoch K.: Lignocellulosic ethanol: Technology design and its impact on process efficiency. Biotechnology Advances. doi:10.1016/j.biotechadv.2014.12.002 (online)

Kolek J., Patakova P., Melzoch K., Sigler K., Řezanka T. Changes in membrane plasmalogens of Clostridium pasteurianum during butanol fermentation as determined by lipidomic analysis. PLOS ONE 10.1371/journal.pone.0122058 (2015)

Patáková P., Linhová M., Vykydalová P., Branská B., Rychtera M., Melzoch K.: Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia. Anaerobe 29: 113-117 (2014), ISSN 1075-9964, IF 2,022 (online)

Paulova L., Hyka P., Branska B., Melzoch K., Kovar K.: Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut(+). Journal of Biotechnology 157 (1): 180-188 (2012). (online)

Patáková P.: Monascus secondary metabolites: production and biological activity. Journal of Industrial Microbiology and Biotechnology 40(2): 169-181. (2013) (online)

Paulová L., Patáková P., Rychtera M., Melzoch K.: High solid fed-batch SSF with delayed inoculation for improved production of bioethanol from wheat straw. Fuel 122: 294-300. (2014) IF 3,406 (online)

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